LOCUS TRUPATH Triple Gaq 10177 bp DNA circular SYN 25-AUG-2024
DEFINITION Encodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit
(GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components
of a BRET2 biosensor for studying heterotrimeric G proteins.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10177)
AUTHORS Olsen RHJ, DiBerto JF, English JG, Glaudin AM, Krumm BE, Slocum ST,
Che T, Gavin AC, McCorvy JD, Roth BL, Strachan RT
TITLE TRUPATH, an open-source biosensor platform for interrogating the
GPCR transducerome.
JOURNAL Nat Chem Biol. 2020 May 4. pii: 10.1038/s41589-020-0535-8. doi:
10.1038/s41589-020-0535-8.
PUBMED 32367019
REFERENCE 2 (bases 1 to 10177)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem
Biol. 2020 May 4. pii: 10.1038/s41589-020-0535-8. doi:
10.1038/s41589-020-0535-8."
FEATURES Location/Qualifiers
source 1..10177
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1937..1990
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
primer_bind complement(3007..3024)
/label=SP6
/note="SP6 promoter, forward primer"
misc_feature 3036..3609
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
misc_feature 3059..3611
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(3203..3220)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 3430..3449
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
primer_bind complement(5768..5785)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5774..5998
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 6044..6472
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6131..6150)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6341..6362
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6481..6501)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 6486..6815
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 6666..6801
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6728..6747
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 6882..7676
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6936..6955)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 7546..7565
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 7850..7971
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7887..7906)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7941..7960
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(8020..8036)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8020..8036)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(8033..8055)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 8044..8060
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8068..8098)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8113..8134
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8251..8268)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8422..9010)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8502..8521)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9181..10041)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9804..9823
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10042..10146)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttatgg gagagatgga gcagctgagg caggaggcag agcagctgaa
961 gaagcagatc gccgacgcca gaaaggcctg cgcagatgtg accctggcag agctggtgag
1021 cggactggag gtggtgggaa gggtgcagat gaggaccagg agaacactga ggggccacct
1081 ggccaagatc tacgccatgc actgggccac agactctaag ctgctggtgt ccgcctctca
1141 ggacggcaag ctgatcgtgt gggatagcta taccacaaac aaggtgcacg caatccccct
1201 gcgcagctcc tgggtcatga cctgtgccta cgccccttcc ggaaatttcg tggcatgcgg
1261 aggactggat aacatgtgct ctatctataa tctgaagagc cgggagggca acgtgaaggt
1321 gtctagagag ctgagcgccc acaccggcta cctgtcttgc tgtcggttcc tggacgataa
1381 caatatcgtg acatctagcg gcgacaccac atgcgcactg tgggacatcg agaccggaca
1441 gcagaagaca gtgtttgtgg gccacacagg cgactgtatg tccctggccg tgtctccaga
1501 tttcaatctg tttatctccg gagcatgcga cgcatctgcc aagctgtggg atgtgcggga
1561 gggcacctgt agacagacct tcacaggcca cgagagcgac atcaacgcca tctgcttctt
1621 tcccaatggc gaggccatct gcacaggcag cgacgatgcc tcctgtaggc tgttcgacct
1681 gcgcgccgat caggagctga tctgcttttc tcacgagagc atcatctgtg gcatcacctc
1741 cgtggccttt agcctgtccg gcaggctgct gttcgcagga tatgacgact tcaactgcaa
1801 cgtgtgggac tccatgaagt ctgagagggt gggcatcctg agcggccacg ataatcgcgt
1861 gtcctgtctg ggagtgaccg cagacggaat ggcagtggcc acaggcagct gggattcctt
1921 tctgaagatc tggaacgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa
1981 tcccggccct accggtatgg tgtccaaggg agaggagctg ttcaccggag tggtgcctat
2041 cctggtggag ctggacggcg atgtgaatgg ccacaagttt agcgtgtccg gagagggaga
2101 gggcgacgca acctacggca agctgacact gaagttcatc tgcaccacag gcaagctgcc
2161 cgtgccttgg ccaaccctgg tgaccacact gtcctacggc gtgcagtgtt tttctaggta
2221 tcctgaccac atgaagcagc acgatttctt taagtctgcc atgccagagg gctacgtgca
2281 ggagcggaca atcttcttta aggacgatgg caactataag accagagccg aggtgaagtt
2341 cgagggcgac acactggtga acaggatcga gctgaagggc atcgacttta aggaggatgg
2401 caatatcctg ggccacaagc tggagtacaa ctataatccc cacaacgtgt atatcatggc
2461 cgataagcag aagaacggca tcaaggtcaa tttcaagatc cgccacaata tcgaggacgg
2521 cagcgtgcag ctggccgatc actaccagca gaacacccca atcggcgacg gacccgtgct
2581 gctgcctgat aatcactatc tgtttacaca gtctgccctg agcaaggacc ccaacgagaa
2641 gcgggatcac atggtgctgc tggagttcgt gaccgcagca ggcatcacac tgggcatgga
2701 tgagctgtac aagggcagcg ccggcaccat ggcacaggac ctgtccgaga aggatctgct
2761 gaagatggag gtggagcagc tgaagaagga ggtgaagaat acaagaatcc ccatcagcaa
2821 ggccggcaag gagatcaagg agtatgtgga ggcccaggcc ggcaacgacc ctttcctgaa
2881 gggcatccca gaggataaga atccctttaa ggagaagggc ggctgcatca tcagctgacc
2941 gcggcgtacg aggcctgcat gctccggacc tgcaggttcg aagtcgacag atctcaattg
3001 gggcccctat agtgtcacct aaataattcc gcccccccct ctccctcccc cccccctaac
3061 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
3121 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
3181 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
3241 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
3301 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
3361 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
3421 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
3481 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
3541 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
3601 cgatgataat atgaccctgg agtccatcat ggcctgctgt ctgtctgagg aggccaagga
3661 ggcccggaga atcaacgacg agatcgagag gcagctgagg agggacaaga gggatgcaag
3721 gagagagctg aagctgctgc tgctgggcac cggagagagc ggcaagtcca ccttcatcaa
3781 gcagatgaga atcatccacg gctctggcta cagcgacgag gataagaggg gcttcaccaa
3841 gctggtgtat cagaatatct ttacagccat gcaggccatg atccgcgcca tggatacact
3901 gaagatccct tacaagtatg agcacaacaa ggcacacgca cagctggtgc gggaggtgga
3961 cgtggagaag gtgtccgcct tctctggagg aggaggcagc atggcctcca aggtgtacga
4021 tcccgagcag agaaagagga tgatcaccgg acctcagtgg tgggcaaggt gcaagcagat
4081 gaacgtgctg gatagcttta tcaattacta tgactccgag aagcacgccg agaacgccgt
4141 gatcttcctg cacggcaatg ccacaagctc ctacctgtgg aggcacgtgg tgccccacat
4201 cgagcctgtg gcaaggtgta tcatcccaga cctgatcggc atgggcaagt ctggcaagag
4261 cggcaatggc agctatcgcc tgctggatca ctacaagtat ctgaccgcct ggttcgagct
4321 gctgaacctg cccaagaaga tcatctttgt gggacacgac tggggagcag cactggcatt
4381 ccactacgcc tatgagcacc aggaccggat caaggccatc gtgcacatgg agtctgtggt
4441 ggatgtgatc gagagctggg acgagtggcc cgacatcgag gaggacatcg ccctgatcaa
4501 gtccgaggag ggcgagaaga tggtgctgga gaacaatttc tttgtggaga ccgtgctgcc
4561 ctctaagatc atgagaaagc tggagcctga ggagttcgcc gcctacctgg agcccttcaa
4621 ggagaagggc gaggtgaggc gccctacact gagctggcca agggagatcc ccctggtgaa
4681 gggcggcaag ccagacgtgg tgcagatcgt gagaaactac aatgcctatc tgagggcctc
4741 cgacgatctg ccaaagctgt tcatcgagag cgatcccggc ttcttttcca acgccatcgt
4801 ggagggcgcc aagaagttcc ccaataccga gtttgtgaag gtgaagggcc tgcactttct
4861 gcaggaggac gcccctgatg agatgggcaa gtacatcaag agcttcgtgg agagagtgct
4921 gaagaacgag cagagcggag gaggaggctc tggagagaat ccatatgtgg atgccatcaa
4981 gtccctgtgg aacgaccccg gcatccagga gtgctacgat cggagaaggg agtatcagct
5041 gtccgactct accaagtact atctgaatga cctggatagg gtggccgacc ctgcctacct
5101 gccaacacag caggatgtgc tgcgcgtgcg ggtgcctacc acaggaatca tcgagtatcc
5161 attcgacctg cagtctgtga tctttcgcat ggtggatgtg ggaggacaga ggagcgagag
5221 gcggaagtgg attcactgtt tcgagaacgt gacatccatc atgtttctgg tggccctgtc
5281 tgagtacgat caggtgctgg tggagagcga caacgagaat agaatggagg agtccaaggc
5341 cctgttcagg accatcatca catacccttg gttccagaac tctagcgtga tcctgtttct
5401 gaataagaag gacctgctgg aggagaagat catgtattcc cacctggtgg attactttcc
5461 cgagtatgat ggacctcaga gagacgcaca ggcagccagg gagttcatcc tgaagatgtt
5521 tgtggacctg aatccagact ctgataagat catctacagc cacttcacct gcgccaccga
5581 cacagagaac atcagattcg tgtttgccgc cgtgaaggat acaatcctgc agctgaacct
5641 gaaggagtat aatctggtgt gaggtaccga gctcggatcc actagtccag tgtggtggaa
5701 ttctgcagat atccagcaca gtggcggccg ctcgagtcta gagggcccgt ttaaacccgc
5761 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
5821 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
5881 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
5941 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
6001 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
6061 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
6121 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
6181 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
6241 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
6301 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
6361 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
6421 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
6481 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
6541 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
6601 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
6661 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
6721 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
6781 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
6841 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
6901 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
6961 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
7021 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
7081 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
7141 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
7201 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
7261 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
7321 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
7381 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
7441 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
7501 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
7561 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
7621 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
7681 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
7741 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
7801 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
7861 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
7921 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
7981 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
8041 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
8101 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
8161 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
8221 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
8281 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
8341 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
8401 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
8461 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
8521 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
8581 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
8641 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
8701 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
8761 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
8821 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
8881 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
8941 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
9001 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
9061 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
9121 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
9181 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
9241 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
9301 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
9361 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
9421 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
9481 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
9541 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
9601 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
9661 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
9721 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
9781 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
9841 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
9901 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
9961 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
10021 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
10081 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
10141 tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
